MIC 206

Transmission of Disease correct answer: 1. Conact 2. Vehicle 3. Vector - Contact between infected and uninfected person - Sexual contact or kissing - Touching, biting or scratching - PLACENTA - Exit or Entry correct answer: Examples of Direct Contact - Pathogen transmitted via inanimate object (fomite) - Needles, toothbrushes, money, diapers, drinking glasses, utensils - Object is contaminated, then uninfected person touches it correct answer: Example of Indirect Contact - ...

Phenylalanine Deaminase Test correct answer: Input(s) = amine group Output(s) = pyruvic acid, NH2 Enzyme(s) = phenylalanine deaminase Reagent(s) = 10% FeCl3 pH Indicator = (+) avocado green, (-) clear Principle of Test: - Identifies organisms that can synthesis the enzyme Phenylalanine Deaminase - Deamination reactions remove the amine (N) groups, producing compounds that are acidic. Starch Hydrolysis Test correct answer: Input(s) = starch Output(s) = glucose Enzyme(s) = amyl...

Simmons Citrate Utilization Test correct answer: Determines the ability of an organism to use sodium citrate as its sole carbon source Simmons Citrate Inputs and Outputs correct answer: Inputs - sodium citrate outputs - basic products Simmons Citrate pH indicator correct answer: Bromthymol Blue Simmons Citrate Enzyme correct answer: Citrate permease Simmons Citrate possible results correct answer: Positive - blue Negative - green Malonate Test correct answer: organism's a...

what is a simple stain and what does it allow for correct answer: A basic stain that is rinsed with water that allows one to identify cell morphology (shape) and arrangement three cell shapes correct answer: bacillus, cocci, spirillum what do stains contain that allow staining to occur correct answer: they contain chromophores, color bearing ions by adding colored compounds in staining, we add ___ for visibility of cells correct answer: contrast charge of bacterial cells correct ...

What is the purpose of staining? correct answer: to add contrast for visibility What are color-bearing ions referred to as? correct answer: chromophores What kind of ions do basic stains have? correct answer: positive charges What is the process called that you do to a MO on a slide before staining it? correct answer: smear prep What are the steps to a smear prep? correct answer: draw circle --> flip and label --> dab 2x loops of MO --> spread --> air dry --> heat...

3 cell shapes correct answer: bacilli, cocci, sprillium by adding colored compound, we add ________ for visibility correct answer: contrast basic stains are __________ correct answer: cations methelyne blue, safranin, and crystal violet are _____ stains correct answer: basic acidic stains are ______ correct answer: anions Heat fix in front of the _____________ for 5 seconds correct answer: bacticinerator when switching to high power and oil power __________ move the coars...

What are the major classes of microorganisms? correct answer: 1. Virus 2. Algae 3. Fungi 4. Protozoa 5. Bacteria What are the two classications of microorgamisms? correct answer: 1. Prokaryotes 2. Eukaryotes What is a viruses classification? correct answer: Viruses are neither prokaryotes or Eukaryotes. What microbe(s) are classified as prokaryotes? correct answer: Bacteria What microbe(s) are classified as Eukaryotes? correct answer: Algae, Fungi, and Protozoa *Nonc...

Microscope photo A correct answer: ocular lens Microscope photo B correct answer: objective lens Microscope photo C correct answer: stage Microscope photo D correct answer: iris diaphragm Microscope photo E correct answer: condensor Microscope photo F correct answer: lamp (light source) Microscope photo G correct answer: arm Microscope photo H correct answer: stage clip Microscope photo I correct answer: fine adjustment knob Microscope photo J correct answer: ...

Required nutrients for microorganisms to grow correct answer: Water, Energy source, Carbon source, Nitrogen, Minerals, Vitamins Chemotrophs correct answer: break chemical bonds to derive energy Phototrophs correct answer: energy derived form sunlight via photosynthetic pigments Hetrotrophs correct answer: Obtain organic carbon (carbs, protein, fats, DNA, amino acids) Autotrophs correct answer: obtain carbon by fixing CO2 States of media correct answer: Liquid, Solid, Semi- s...

ability to transfer microbes from one place to another without contaminating the original culture, the new medium or the environment correct answer: define aseptic technique - disinfecting workbenches before and after use/washing hands - sterilizing media and inoculating instruments - transfer of microbes without contamination - pinky finger hold of test tube cap, tilting the test tube to minimize air contaminants, working efficiently to reduce to exposure to air, etc. correct answer: lis...