Lab practical bio 255 Samenvattingen, Aantekeningen en Examens
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MICROBIOLOGY LAB PRACTICAL 2 VERIFIED ANSWERS UPDATED 2024
- Samenvatting • 4 pagina's • 2024
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- $5.99
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Selective and Differential plates: 
EMB: 
Gram negative rods grow 
Uses dyes eosin Y and methylene blue to inhibit Gram positive 
Gram negative rods are differentiated based on their ability of ferment lactose 
Slower fermenters: pink 
Medium fermentors: purple 
Fast fermentors: green 
Non-fermenters: light, translucent color 
MAC: 
Gram negative rods grow 
Uses crystal violet and bile salts to inhibit Gram positive 
Gram negative rods are differentiated based on their ability
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MICROBIOLOGY LAB PRACTICAL 1 VERIFIED RATIONALES LATEST 2024/2025
- Tentamen (uitwerkingen) • 3 pagina's • 2024
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- $6.99
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Gram Stain 
Positive: Purple 
Negative: Pink 
1. 4-5 layers 
2. Crystal Violet 
3. Iodine 
4. Decolorizer 
5. Safrin 
2. Bacteria Shape 
Bacillus: rod shaped 
Coccus: round shape 
Spiral: spiral shaped 
3. Catalase Test 
Hydrogen peroxide is toxic to bacteria and may be produced in the cell. Some bacteria contain 
catalase which can detoxify the hydrogen peroxide by converting it to water and gaseous oxygen. 
Positive: Bubbles 
Negative: Nothing happens 
Oxygen gas comprises the bubbles 
4. Oxid...
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