RFLP Study guides, Class notes & Summaries

What does barcoding do in Next Generation Sequencing? Adds unique tag to all nucleic acids within one sample so that various samples can be mixed What is the best way to store RNA for longer than a month? In Ethanol, at -20°C or -80°C Testing for Aldehyde Dehydrogenase deficiency is important in patients with: Alcohol dependence During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an incoming nucleotide? Beta The 5' capping pr...

Consider the table below where a child and a possible father (PF) share the listed paternity indices for each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4). Locus Tested PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 15/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table, what is the combined paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4? 12.42 9.558 15.56 2.38 - ANSWER 12.42 ( CPI is calculated by multiplying ...

What is metagenome? - correct answer Sampling the genome sequence of a community of organisms. Polymorphism involved for Irinotecan? - correct answer UGT1A1 NGS related to deletions and insertions, coverage, depth? - correct answer -Paired-end reads for detection of indels -Coverage is how much of your target has been read -Depth is how many times a...

MB (ASCP) Practice Exam Questions and Answers 2023 What does barcoding do in Next Generation Sequencing? Adds unique tag to all nucleic acids within one sample so that various samples can be mixed What is the best way to store RNA for longer than a month? In Ethanol, at -20°C or -80°C Testing for Aldehyde Dehydrogenase deficiency is important in patients with: Alcohol dependence During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an ...

Biology Exam 3 Practice Test 40 Questions with Verified Answers In the 1940s and 1950s scientists were performing experiments to discover what molecule was the inheritance material. The basis of that work was Griffith's transformation experiments with the rough and smooth strains of Pneumococcus. Rough bacterial strains were transformed by A. treatment with radiation. B. infection with a virus. C. uptake of protein from the environment. D. uptake of DNA from the environment. - CORRECT...

Restriction endonuclease - -cleaves DNA molecules at a specific sequence of bases à molecular scissors, Laboratory tools, wide spectrum of application. They have sticky and blunt ends. -Enzymes Used in DNA Technology - -I. Restriction endonuclease -cleaves DNA molecules at a specific sequence of bases II. DNA ligase -joins DNA strands together III. DNA polymerase -makes DNA from nucleotides -Taq DNA polymerase IV. Reverse transcriptase -generates complementary DNA (cDNA) from an RNA ...

MB (ASCP) Review & Exam Questions Part III latest updatePCR can test for what diseases? Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus qPCR can test for what diseases? Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 00:49 01:08 RT PCR can test for what diseases? HIV Influenza Coronavirus Ewing sarcoma Synovial sarcoma Hep -...

OpenStax Microbiology Test Bank Chapter 12: Modern Applications of Microbial Genetics Chapter 12: Modern Applications of Microbial Genetics * = Correct answer Multiple Choice 1. Which of the following is the definition of biotechnology? A. The alteration of DNA in a cell B. The use of living systems to benefit humankind* C. Using computers to synthesize biomolecules D. Using DNA for solving mathematical problems Difficulty: Easy ASM Standard: 26 2. Which of the following is the defin...

PPE - ANSWER-Personal protective equipment v HIPAA - ANSWER-(Health Insurance Portability and Accountability Act) - Regulates how medical professionals and other people use and disclose certain individually identifiable health information. Times when patient's confidentiality should be broken... - ANSWER-if it was a minor(18 and younger), causes dangers to other Steps for processing a crime scene - ANSWER-Sketch, photograph, interview, collect evidence, and examine the scene 5 Types...

MB (ASCP) Review & Exam Questions Part II Questions and answers 2023 What is the Beer-Lambert Law? the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: 260nm Proteins are absorbed at: 280nm What is the melting temperature equation? Tm = (4 x # of GC pairs) + (2 x # of AT p...